Structure of the native microtubule lattice nucleated from the yeast spindle pole body
Resolution: 6.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv0
Q-score: 0.123
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ Pubmed: 38609662 DOI: doi:10.1038/s41594-024-01281-y ]
Resolution: 9.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv2
Q-score: 0.112
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ Pubmed: 38609662 DOI: doi:10.1038/s41594-024-01281-y ]
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Unkown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
- Y-tubulin ring complex capping the microtubule minus end (Cellular component from Saccharomyces cerevisiae)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Water (18 Da, Ligand)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 8.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv3
Q-score: 0.144
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ Pubmed: 38609662 DOI: doi:10.1038/s41594-024-01281-y ]
- Y-tubulin small complex bound to two alpha/beta tubulin dimers (Cellular component from Saccharomyces cerevisiae)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
Structure of the human outer kinetochore KMN network complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ppr
Q-score: 0.341
Yatskevich S, Yang J, Bellini D, Zhang Z, Barford D
Nat Struct Mol Biol (2024) [ Pubmed: 38459127 DOI: doi:10.1038/s41594-024-01249-y ]
- Kinetochore protein spc24 (22 kDa, Protein from Homo sapiens)
- Kinetochore-associated protein dsn1 homolog (40 kDa, Protein from Homo sapiens)
- Polyamine-modulated factor 1 (23 kDa, Protein from Homo sapiens)
- Outer kinetochore kmn network junction complex (Complex from Homo sapiens)
- Kinetochore scaffold 1 (265 kDa, Protein from Homo sapiens)
- Zw10 interactor (31 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Kinetochore-associated protein nsl1 homolog (32 kDa, Protein from Homo sapiens)
- Kinetochore protein spc25 (26 kDa, Protein from Homo sapiens)
- Protein mis12 homolog (24 kDa, Protein from Homo sapiens)
Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovw
Q-score: 0.386
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
Cryo-EM structure of yeast CENP-OPQU+ bound to the CENP-A N-terminus
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovx
Q-score: 0.283
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ow0
Q-score: 0.2
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ow1
Q-score: 0.165
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
Multibody map of the CENP-A nucleosome as part of the inner kinetochore
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
Multibody map of CCAN(Topo) bound to CON3 DNA as part of the Inner kinetochore.
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]